深圳地区艾滋病病毒感染人群的HIV-1抗性基因多态性研究Polymorphisms of HIV - 1 Resistance Alleles in HIV - 1 Carriers in Shenzhen City of China
王福生,金磊,侯静,施红,洪卫国,雷周云,王业东,冯铁建,王晓辉,陈琳,李良成
摘要(Abstract)：
目的 研究HIV-1相关的等位基因CCR5△32、CCR5m303、CCR2b和SDF1在艾滋病病毒感染人群中的分布,为深入分析这些遗传突变在HIV-1感染、艾滋病发病进程中的作用提供理论依据。方法 收集深圳地区近年来艾滋病病毒感染者的血液标本共91份,提取基因组DNA。经PCR或PCR-RFLP分析,计算遗传突变频率、纯合子和杂合子的构成比例。采用x2检验或t检验进行群体分布的拟合度和差异的显著性分析。结果 在所有被检测的91例HIV-1感染者的个体中,未发现有CCR5△32和CCR5m303的遗传突变,CCR2b-64I和SDF1-3＇A的突变频率较高,分别为21.43％和25.82％。与中国普通汉族人的结果相近。结论 研究结果提示深圳地区的艾滋病病毒感染者本身对HIV-1病原体可能具有较大的遗传易感性。由于他们具有较高的CCR2b-64I和SDF1-3＇A突变频率,后者在艾滋病发病过程中的影响值得进一步研究。
关键词(KeyWords)： HIV-1;遗传易感性; 基因多态性; 协同受体; 性传播疾病
基金项目(Foundation): 国家自然科学基金资助课题（397706830）
作者(Author): 王福生,金磊,侯静,施红,洪卫国,雷周云,王业东,冯铁建,王晓辉,陈琳,李良成
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